Thingiverse
Autostainer for Biological Specimens 3D Printed USB Powered by sandrabaskharon
by Thingiverse
Last crawled date: 3 years ago
3D printing is considered a transformational technology; however, its potential for configuring research laboratory equipment is nearly unprecedented. This project uses inexpensive and widely available electronic parts and a commercially available 3D printer to create a USB Powered Autostainer for Biological Specimens.
This platform uses two USB powered stepper motors (one turns the stain tanks into position under the slide and one dips the slide holding biological material into the stain tank), two stepper motor Driver boards, one Arduino UNO microcontroller, one LCD Shield and custom designed ABS plastic 3D printed structural components.
The microcontroller program polls buttons on the Shield to write a 5 step staining protocol. It is fully programmable from this LCD Shield user interface and does not need to be connected to a computer for program control. Each step is written into an element of an array for later program execution. The program first "Homes" the Dip and then the Tank stepper motor against limit switches. The program then executes each movement from the arrays which received the programming steps.
Future developments include inexpensive autostainer platforms for any high school program with access to a 3D printer as well as additional modules for the platform for example an auto-immunostainer or a Thermofoil heated Real Time PCR platform. Since the platform uses only USB power is conceivable that it could be powered by a small solar panel (for field research).
Automated Laberatory Equiptment
Automated systems are essential in both education and in research.
Essentially all laboratory equipment uses similar components (optics, fluidics, controlled movements, pneumatics and data management
Using 3D printed parts and frames allows you to rapidly prototype low cost automated laboratory equipment for Educational uses and for field research.
Being able to control the movements of automated systems in research or in education can be a tremendous advantage for creating automated systems
Using USB power allows lower power consumption (USB 4.5 V and 100 mA)
3D printed prototype platforms can be Scalable, Modular and Expandable
Stain for Testing the System
Diff-Quik is a commercial Romanowsky stain variant, commonly used in histological staining to rapidly stain and differentiate a variety of smears, commonly blood and non-gynecological smears, including those of fine needle aspirates. It is based on a modification of the Wright Giemsa stain pioneered by Bernard Witlin in 1970. It has advantages over the older Wright Giemsa staining technique, as it reduces the 4 minute process into a simplified 15 second operation, and allows for selective increased eosinophilic or basophilic staining depending upon the time the smear is left in the staining solutions.
Diff-Quik is utilized on material which is air-dried prior to alcohol fixation .
Solutions required:
Fixative (Fast green in methanol) - pale green colour
Stain solution 1 (Eosin G in phosphate buffer) - red colour
Stain solution 2 (Thiazine dye in phosphate buffer) - blue colour
Method:
Allow smears to dry
Dip slide five times, for one second each, into Fixative.
Allow excess to drain after each dip.
Dip slide or tape-strip five times, for one second each, into Stain
Configuration:
A portable Up!3D printer was used to fabricate the 3D printed parts. The printer used the following parameters:
Z Resolution of 0.20mm, Base height 2 mm, Support Density 2 layers, 25% infill of parts interiors. The parts were printed using ABS plastic filament obtained form Octave.com. The parametric files for each object were saved in standard STL (stereolithography) file.
The Arduino UNO Microcontroller was selected because it permitted an adequate number of digital inputs and outputs to control this prototype while allowing expansion for future development of related systems.
The stepper motors needed to run with adequate force using USB power. The Steppers were wired as Bipolar motors and the Sparkfun Bipolar motor driver permitted control with the Arduino microcontroller.
The entire system was designed for low cost (<50$), reliability, low power consumption and portability.
Limitations:
Platform limited to staining a single slide staining at a time
This version is limited to 4 staining reservoirs or wells, maximum
There is no blotting pad or wipe between the staining dips
Changes in the microcontroller program require computer connection
The housing for the unit needs to protect the wiring
The electronics should be embedded in a cowling or cover
There is currently no way to change the distance that the slide travels
3D printed parts:
Slide guide -ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ guides glass slide
Limit switch holder (Tank) - Snap-Arm
Limit switch holder (Slide Dip) proximityÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ
Support wire guide - supports microscope slide
Arch - Rotates to Lower glass slide for staining
Stepper holder (Tank) rotates stain reservoirs
Tank rotation base, attached to Mentos holder
Reservoir holder for Mentos containers
FUture Platform Enhancements:
This flexible platform is:Automated, reliable, programmable, scalable, modular, expandable, inexpensive, with low power consumption, a small footprint and is assembled with 3D printed parts.
The next generation may include:
Auto Immunohistochemistry platform, by adding three syringe pumps.
In situ hybridization platform adding USB powered thermofoil heaters.
Linked modules to support more complex staining protocols
Labview control interface or Scratch S4A for more flexible control
This High school science project was overseen by:
Dr. R. Siderits at RWJUHH:
Awards
1) Office of Naval Research Naval Science Award Certificate and a medal
2) AMS Materials Education Foundation for Most Outstanding Exhibit in Materials Science
3) First Place in the Senior division in the field of Engineering Materials and Bioengineering
4) Technology Grant in the form of a microcontroller system called a Beagle board.
This platform uses two USB powered stepper motors (one turns the stain tanks into position under the slide and one dips the slide holding biological material into the stain tank), two stepper motor Driver boards, one Arduino UNO microcontroller, one LCD Shield and custom designed ABS plastic 3D printed structural components.
The microcontroller program polls buttons on the Shield to write a 5 step staining protocol. It is fully programmable from this LCD Shield user interface and does not need to be connected to a computer for program control. Each step is written into an element of an array for later program execution. The program first "Homes" the Dip and then the Tank stepper motor against limit switches. The program then executes each movement from the arrays which received the programming steps.
Future developments include inexpensive autostainer platforms for any high school program with access to a 3D printer as well as additional modules for the platform for example an auto-immunostainer or a Thermofoil heated Real Time PCR platform. Since the platform uses only USB power is conceivable that it could be powered by a small solar panel (for field research).
Automated Laberatory Equiptment
Automated systems are essential in both education and in research.
Essentially all laboratory equipment uses similar components (optics, fluidics, controlled movements, pneumatics and data management
Using 3D printed parts and frames allows you to rapidly prototype low cost automated laboratory equipment for Educational uses and for field research.
Being able to control the movements of automated systems in research or in education can be a tremendous advantage for creating automated systems
Using USB power allows lower power consumption (USB 4.5 V and 100 mA)
3D printed prototype platforms can be Scalable, Modular and Expandable
Stain for Testing the System
Diff-Quik is a commercial Romanowsky stain variant, commonly used in histological staining to rapidly stain and differentiate a variety of smears, commonly blood and non-gynecological smears, including those of fine needle aspirates. It is based on a modification of the Wright Giemsa stain pioneered by Bernard Witlin in 1970. It has advantages over the older Wright Giemsa staining technique, as it reduces the 4 minute process into a simplified 15 second operation, and allows for selective increased eosinophilic or basophilic staining depending upon the time the smear is left in the staining solutions.
Diff-Quik is utilized on material which is air-dried prior to alcohol fixation .
Solutions required:
Fixative (Fast green in methanol) - pale green colour
Stain solution 1 (Eosin G in phosphate buffer) - red colour
Stain solution 2 (Thiazine dye in phosphate buffer) - blue colour
Method:
Allow smears to dry
Dip slide five times, for one second each, into Fixative.
Allow excess to drain after each dip.
Dip slide or tape-strip five times, for one second each, into Stain
Configuration:
A portable Up!3D printer was used to fabricate the 3D printed parts. The printer used the following parameters:
Z Resolution of 0.20mm, Base height 2 mm, Support Density 2 layers, 25% infill of parts interiors. The parts were printed using ABS plastic filament obtained form Octave.com. The parametric files for each object were saved in standard STL (stereolithography) file.
The Arduino UNO Microcontroller was selected because it permitted an adequate number of digital inputs and outputs to control this prototype while allowing expansion for future development of related systems.
The stepper motors needed to run with adequate force using USB power. The Steppers were wired as Bipolar motors and the Sparkfun Bipolar motor driver permitted control with the Arduino microcontroller.
The entire system was designed for low cost (<50$), reliability, low power consumption and portability.
Limitations:
Platform limited to staining a single slide staining at a time
This version is limited to 4 staining reservoirs or wells, maximum
There is no blotting pad or wipe between the staining dips
Changes in the microcontroller program require computer connection
The housing for the unit needs to protect the wiring
The electronics should be embedded in a cowling or cover
There is currently no way to change the distance that the slide travels
3D printed parts:
Slide guide -ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ guides glass slide
Limit switch holder (Tank) - Snap-Arm
Limit switch holder (Slide Dip) proximityÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ
Support wire guide - supports microscope slide
Arch - Rotates to Lower glass slide for staining
Stepper holder (Tank) rotates stain reservoirs
Tank rotation base, attached to Mentos holder
Reservoir holder for Mentos containers
FUture Platform Enhancements:
This flexible platform is:Automated, reliable, programmable, scalable, modular, expandable, inexpensive, with low power consumption, a small footprint and is assembled with 3D printed parts.
The next generation may include:
Auto Immunohistochemistry platform, by adding three syringe pumps.
In situ hybridization platform adding USB powered thermofoil heaters.
Linked modules to support more complex staining protocols
Labview control interface or Scratch S4A for more flexible control
This High school science project was overseen by:
Dr. R. Siderits at RWJUHH:
Awards
1) Office of Naval Research Naval Science Award Certificate and a medal
2) AMS Materials Education Foundation for Most Outstanding Exhibit in Materials Science
3) First Place in the Senior division in the field of Engineering Materials and Bioengineering
4) Technology Grant in the form of a microcontroller system called a Beagle board.
Similar models
thingiverse
free
Filament guide replacement for Eventorbot direct extruder by Sungod3k
...re. this one a problem in the first version (http://www.thingiverse.com/thing:172076) and should be substantially reduced by now.
thingiverse
free
Integrated Spool Holding System for the Felix Robotics Printer by Pacificimc
...ãâãâ¢ãâãâãâãâãâãâãâãâsolid and very little filament resistanceãâãâãâãâ¢ãâãâãâãâãâãâãâãâ
thingiverse
free
coremic by mic
...tepper con planetario dell'asse z per questo tipo di stampanti ( esistino altri tipi di stampanti con movimenti di z simili)
grabcad
free
linear slide stepper motor with l2 98 driver
... stepper motor with l2 98 driver
grabcad
a linear moving system using linear slide stepper motor with a l2 98 powered by battery
thingiverse
free
MobyStick - Whale USB and SD holder by TwistedDaylight
...wanting to make their own stuff.
06.02.14 updated the files, now the sd and usb slots have 0,4mm tolerance to allow proper fit.
blendswap
free
pearHI
... ãâ¬ãâµãâ°ãâ»ãâ¸ãâãâãâ¸ãâ¡ãâ½ãâ¾ãâ¹.latest change on 2013/04/19, 08:58:52ðºð¾ññðµðºñð¸ñð¾ð²ðºð° ñðµðºññð°
thingiverse
free
Shrek (The Ogrelord) ( áð ÃÂàáð) ( áð ÃÂàáð) ( áð ÃÂàáð) ( áð ÃÂàáð) by Badfish2
...â ãâ¡ãâ°) ( ãâ¡ãâ° ãâãâ ãâ¡ãâ°) by badfish2
thingiverse
shrek is love. shrek is life. ( ãâ¡ãâ° ãâãâ ãâ¡ãâ°)
blendswap
free
2014 newyear forest
...¹ãâ¹ ãâ¸ãâ½ãâãâãâ¬ãæãâ¼ãâµãâ½ãâ, ãâ²ãâãâµãâ¼ ãâºãâãâ¾ ãâµãâ³ãâ¾ ãâãâ¾ãâ·ãâ´ãâ°ãâ²ãâ°ãâ».
thingiverse
free
Blade-1ã¯ã¼ãªã³ã°ãÂÂã¡ã³ãÂÂãºã« by Hotproceed
...â°ã£ââã£ââ¡ã£ââ³ã£ââã£ââã£ââã£ââ«ã¥â
â¥ã£ââ£ã£ââ¦ã£ââã£ââã£ââã£ââºã£ââ«ã£ââã£ââ¼ã£ââ¿ã£ââ§ã£ââã£ââ
thingiverse
free
N-DO badge by hare081987
...n-do badge by hare081987
thingiverse
ã â¸âã â¸â£ã â¸â²ã â¸âã â¸â±ã â¹âã â¸â¡ã â¹âã â¸âã â¹âã â¸âã â¸âã â¸â¹
Specimens
turbosquid
$5
specimen#592
... available on turbo squid, the world's leading provider of digital 3d models for visualization, films, television, and games.
turbosquid
$11
RH BOTANICAL SPECIMEN COLLECTION
...al specimen collection for download as 3ds, max, obj, and fbx on turbosquid: 3d models for games, architecture, videos. (1194764)
turbosquid
free
Sci Fi Specimen Tank
... available on turbo squid, the world's leading provider of digital 3d models for visualization, films, television, and games.
turbosquid
$100
Burchells zebra (Animated) Museum Specimen
...chells zebra (animated) museum specimen for download as blend on turbosquid: 3d models for games, architecture, videos. (1319094)
turbosquid
$89
Buck Museum Specimen (Rigged)(Fur)
... available on turbo squid, the world's leading provider of digital 3d models for visualization, films, television, and games.
turbosquid
$100
Giraffe Museum Specimen (Animated)
...
royalty free 3d model giraffe for download as obj and blend on turbosquid: 3d models for games, architecture, videos. (1270339)
turbosquid
$49
Specimen with half human and half shark/dolphin anatomy
... available on turbo squid, the world's leading provider of digital 3d models for visualization, films, television, and games.
turbosquid
$89
Southern White Rhino Museum Specimen (Animated)
...el southern white african rhino for download as obj and blend on turbosquid: 3d models for games, architecture, videos. (1262481)
design_connected
free
Sarn
...sarn
designconnected
free 3d model of sarn by specimen editions designed by thinkk studio.
design_connected
$18
Weight Vases
...weight vases
designconnected
specimen editions weight vases computer generated 3d model. designed by ecal.
Biological
turbosquid
$29
Bag Biological Waste
... biological waste for download as max, max, 3ds, fbx, and obj on turbosquid: 3d models for games, architecture, videos. (1636267)
turbosquid
$10
Radioactive/Biological/Flammable Barrels
... available on turbo squid, the world's leading provider of digital 3d models for visualization, films, television, and games.
3d_export
$10
House in natural style
...house in natural style 3dexport biologicalstyle apartment building connecting architecture and...
3d_export
$5
Cauble neytral rug
...neutral rug from crate & barrel. comfortable, soft, fluffy, biologicaly clean...
3d_export
$5
download 3d picture of ear picture of ear
...3d model picture of ear, model of ear, human biological ear no watermark hd ear image...
3d_export
$39
Exterminator 3D Model
...uniform coveralls protection man mask gas respirator insect gasmask biological toxicpoison chemical characters bilogical toxin human exterminator 3d model...
3d_export
$15
spacecraft foton-m
...experiments in orbital flight, the production of materials and biological preparations in the interests of various industries and science,...
3d_export
$39
Man In Protective Hazmat Suit 3D Model
...low poly game niform coveralls protection gas respirator insect biological man in protective hazmat suit 3d model beatheart 99915...
cg_studio
$13
Shell barrel3d model
...warehouse storage factory set pack barrel barrels pallet container biological rusty oil 3d model vray gasoline petrol gas shell...
3d_ocean
$29
Exterminator
...exterminator 3docean bilogical biological bug bugs character characters chemical coveralls exterminator gas gasmask...
Usb
3d_ocean
$3
USB Thumbdrive
...usb thumbdrive
3docean
thumbdrive usb usb drive usb stick
this is a swivel type usb thumb drive.
3d_ocean
$5
Usb Stick
...usb stick
3docean
flash stick usb usb memory usb stick
usb stick created using 3ds max 2015 and rendered on mental ray.
3ddd
$1
USB cable
... кабель , провод
высокополигональные модели кабелей usb.
3d_ocean
$5
USB Stick
...usb stick
3docean
32gb computer memory plug plugin protection usb usb stick
an usb stick with built-in plug protection
3d_ocean
$6
USB Stick
...tick
3docean
32 gb flash ram gigabyte memory metal nand plug ram stick usb usb 3 usb stick
usb stick with texture and normal map.
turbosquid
$5
USBS
...bs
turbosquid
royalty free 3d model usbs for download as max on turbosquid: 3d models for games, architecture, videos. (1466594)
3d_export
$5
usb flash
...usb flash
3dexport
usb flash
3d_export
free
usb flash
...usb flash
3dexport
usb flash
3d_export
free
usb flash
...usb flash
3dexport
usb flash driver
3ddd
$1
usb
...usb
3ddd
флешка
флешка
Powered
turbosquid
$100
power
...ower
turbosquid
royalty free 3d model power for download as on turbosquid: 3d models for games, architecture, videos. (1421990)
3d_export
$5
Power
...power
3dexport
3d_export
$5
power outlets
...power outlets
3dexport
power outlets
3ddd
$1
lion power
...lion power
3ddd
лев , статуя
lion power gold sculpture
3ddd
$1
Sea Power
...
компас , море , часы
часы с компасом sea power
3ddd
free
Meridiani / Power
...power
3ddd
meridiani , круглый
стол power производитель meridiani, диаметр 120,высота 67
3d_export
$5
Power Surge
...power surge
3dexport
the power surge is a all mesh carnival ride to lower in game part count and lag
turbosquid
$8
Airport Ground Power Unit (AXA Power )
... available on turbo squid, the world's leading provider of digital 3d models for visualization, films, television, and games.
turbosquid
$50
Power Houser
...rbosquid
royalty free 3d model power houser for download as on turbosquid: 3d models for games, architecture, videos. (1333800)
3d_export
$5
power outlet
...power outlet
3dexport
power outlet<br>format file maya 2018, 3d max 2017, obj, fbx
Printed
design_connected
$27
...print
designconnected
moroso print computer generated 3d model. designed by wanders, marcel.
3ddd
free
Eichholtz Prints
...- eichholtz print central station i
13 - eichholtz print central station ii
14 - eichholtz print marisa
15 - eichholtz print tish
3ddd
$1
Eichholtz Prints
...print abstract - set of 2
10 - eichholtz print orange abstract
11 - eichholtz print buddha right
12 - eichholtz print buddha left
turbosquid
$1
... available on turbo squid, the world's leading provider of digital 3d models for visualization, films, television, and games.
3ddd
free
Eichholtz Prints
...of 4
2 - print dunbar 2 set of 4
3 - print guadeloupe 1 set of 4
4 - print guadeloupe 2 set of 4
5 - print giles
6 - print trett
3ddd
$1
Eichholtz Prints
...nt tutti frutti
3 - eichholtz prints watson - set of 2
4 - eichholtz prints antique nautilus - set of 2
5 - eichholtz print tiara
3d_export
$5
Monster for printing
...monster for printing
3dexport
monster 3d model printing
3ddd
free
printed rug
...printed rug
3ddd
ковер
very creative printed rug
3ddd
free
Eichholtz Prints
...иал: бумага
габариты (вхш): 72 x 62 см
описание: print sweetmeat - постер в деревянной раме.
3 - prints varsity set of 2
арти
3ddd
free
Art Print Posters
...art print posters
3ddd
прованс
art print posters by patrician prints