APAF-1 & Caspase-9 Complex (PDB 4RHW) by sokamarintchak 3d model
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APAF-1 & Caspase-9 Complex (PDB 4RHW) by sokamarintchak

APAF-1 & Caspase-9 Complex (PDB 4RHW) by sokamarintchak

by Thingiverse
Last crawled date: 2 years, 12 months ago
Imaged is my first printout of one-half of the PDB 4RHW molecule (APAF-1 & Caspase-9 complex). The printout is very rigid adding in reinforcing connections. After printing, a few unstable locations were identified. The main weak point being the chloride atom found near the center of the molecule. Additional reinforcing connections were added and included within the provided file.
The APAF-1 is a key player in the execution of apoptosis, or programmed cell death. Oligomerization of 7 APAF-1 molecules a structure called the "apoptosome" forms. The apoptosome converts pro-caspase-9 to caspase-9 which is important for the activation of cellular proteases involved in apoptosis. This protein molecule is interesting in that it only contains alpha-helices.
The original PDB file (4RHW: http://www.rcsb.org/pdb/explore/explore.do?structureId=4RHW) was manipulated using VMD (http://www.ks.uiuc.edu/Research/vmd/) to remove water and sulfate molecules. The protein in the PDB file is a dimer so half the protein was isolated and represented. The structural representation in VMD was then set to NewCartoon and exported out of VMD as an .STL file.
The .STL file was imported into Tinkercad (https://www.tinkercad.com/). Within Tinkercad, reinforcing braces and supports were added manually. The reinforcing braces between the alpha-helices are meant to be left within the molecule to increase structural rigidity whereas the base supports were added to facilitate the printing process.

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