96 well to 384 well pipetting guide by daj1u06

Last crawled date: 4 years, 9 months ago

This came about when the pipetting robot in the local microbiology lab involved in COVID testing broke down, requiring the team to manually consolidate the contents of 4 96 well plates in a consistent, error-free and known pattern into a 384 well plate for the next step of the assay. The jig sits on the 384 well plate with position A at top left. and the 96 holes allow a regular subset of the wells to be filled using 8 or 12 channel pipettes. The jig is then rotated 180 degrees in the horizontal plane so B is at top left, exposing another set of 96 wells. It is then flipped right to left so C is at top left to expose the 3rd set, then rotated 180 degrees in the horizontal plane again so D is at top left. The freecad file has the 384 well centres, and the middle of a corner well as reference objects. It requires support for printing so a dual channel printer is needed. Thanks to Simon Friar from the local PHE COVID team for the suggestion of flipping a plate, rather than printing 4 different plates.